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( a ) Analysis of His-rGP5 and rGP5-FljB with an anti-PRRSV polyclonal antibody. Lanes: M, molecular weight markers; 1, 1 × SDS-loading buffer; 2, purified His-rGP5 protein; 3, …

Flagellin fljB gene (light blue) was amplified from the genomic DNA of attenuated Salmonella Typhimurium SL7207 strain and cloned into the Eco RI and Sal I sites of pCold-rGP5, resulting in a ...

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The IgG1/IgG2a ratio in the rGP5-FljB immunization group was significantly higher (9-fold) than that in the rGP5 alone group and was equivalent to the response in the rGP5 + Alum immunization ...

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Mosaic DNA and vaccinia (VACV) vaccines were developed in order to improve protection against heterologous PRRSV strains. Methods: Piglets were primed …

Cells were treated with endotoxin-free recombinant proteins rGP5 or rGP5-FljB at the concentration of 10 and 100 ng/ml for 5 h. For positive controls, HEK293-mTLR5 cells were treated with the TLR5 ...

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Schematic representation for the construction of rGP5 and rGP5-fljB. rGP5 fragment with the deletion of its signal peptide (green) and transmembrane regions (light grey) was amplified by overlap-PCR, and inserted into the BamHI and EcoRI digested expression vector pColdI or pGEX-6p-1 to create pCold-rGP5 or pGEX-6p-1-rGP5 …

The results show that immunization with the fusion protein rGP5-FljB induced a significantly enhanced GP5-specific and PRRSV-specific IgG response that …

Porcine reproductive and respiratory syndrome (PRRS) is characterized by severe reproductive failure and severe pneumonia in neonatal pigs and is caused by …

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Rapid Growth Project 5, Yandi Mine. Hanson's Yandi mobile plant in Western Australia has produced concrete for BHP Billitons RGP5 at the Yandi mine since 2001. RGP5 is part of Billiton's iron ore expansion in Western Australia's Pilbara region. A new plant was constructed as part of RGP5 to increase installed capacity across BHP Billion ...